Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[2H0] ratios higher than in AA. At high AA concentration preferential conversion to elongated fatty acids with 5 unsaturations, through a 6 desaturase takes place and the 4-desaturated 22:5 appears to be formed through beta-oxidation of 24:5.

Formation of 22 and 24 carbon 6-desaturated fatty acids from exogenous deuterated arachidonic acid is activated in THP-1 cells at high substrate concentrations / D. Caruso, P. Risé, G. Galella, C. Regazzoni, A. Toia, G. Galli, C. Galli. - In: FEBS LETTERS. - ISSN 0014-5793. - 343:3(1994 May 02), pp. 195-9-199.

Formation of 22 and 24 carbon 6-desaturated fatty acids from exogenous deuterated arachidonic acid is activated in THP-1 cells at high substrate concentrations

D. Caruso;P. Risé;A. Toia;G. Galli;C. Galli
1994-05-02

Abstract

Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[2H0] ratios higher than in AA. At high AA concentration preferential conversion to elongated fatty acids with 5 unsaturations, through a 6 desaturase takes place and the 4-desaturated 22:5 appears to be formed through beta-oxidation of 24:5.
4 Desaturation in 22:5 n-6; 6 Desaturation of 24:4 n-6; Deuterated 20:4 n-6, GC-MS; THP-1 cell
Settore BIO/10 - Biochimica
Settore BIO/14 - Farmacologia
FEBS LETTERS
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/197823
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