OBJECTIVE: To identify beta-adrenergic receptor subtypes in ileum smooth muscle of the horse. SAMPLE POPULATION: Isolated strips of equine longitudinal ileum smooth muscle and membrane preparations from smooth muscle of the intestinal wall. PROCEDURE: Functional assays and radioligand binding assays. RESULTS: Relaxation of ileum longitudinal smooth muscle proved to be mainly caused by stimulation of beta-atypical and beta 2-adrenergic receptors. Binding studies on cell membranes indicated that the total beta-adrenergic receptors population consists of 54% beta-atypical, 34% beta 2- and 12% beta 1-subtypes. CONCLUSIONS: The data suggest that sympathetic relaxation of equine ileum smooth muscle depends mainly on beta-atypical receptor subtypes activation, with a minor contribution by beta 2-subtypes. CLINICAL RELEVANCE: The important role of beta-atypical adrenergic receptor subtypes in the relaxation of equine ileum suggests possible clinical use of selective beta-atypical receptor agonists to control intestinal disturbances.
Identification of ß-adrenergic receptor subtypes mediating relaxation in isolated equine ileum / G. Re, C. Belloli, P. Badino, F. Arioli, A. Novelli, C. Girardi, C. Beretta. - In: AMERICAN JOURNAL OF VETERINARY RESEARCH. - ISSN 0002-9645. - 58:6(1997 Jun), pp. 621-625.
Identification of ß-adrenergic receptor subtypes mediating relaxation in isolated equine ileum
F. Arioli;C. Beretta
1997
Abstract
OBJECTIVE: To identify beta-adrenergic receptor subtypes in ileum smooth muscle of the horse. SAMPLE POPULATION: Isolated strips of equine longitudinal ileum smooth muscle and membrane preparations from smooth muscle of the intestinal wall. PROCEDURE: Functional assays and radioligand binding assays. RESULTS: Relaxation of ileum longitudinal smooth muscle proved to be mainly caused by stimulation of beta-atypical and beta 2-adrenergic receptors. Binding studies on cell membranes indicated that the total beta-adrenergic receptors population consists of 54% beta-atypical, 34% beta 2- and 12% beta 1-subtypes. CONCLUSIONS: The data suggest that sympathetic relaxation of equine ileum smooth muscle depends mainly on beta-atypical receptor subtypes activation, with a minor contribution by beta 2-subtypes. CLINICAL RELEVANCE: The important role of beta-atypical adrenergic receptor subtypes in the relaxation of equine ileum suggests possible clinical use of selective beta-atypical receptor agonists to control intestinal disturbances.Pubblicazioni consigliate
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