• Aureobasidium pullulans LV 10 produced extracellular pectolytic activity when grown in a medium containing apple pectin as a carbon source. Maximum enzyme production (22 PGU ml−1 and 9 PLU ml−1) was obtained after 4 days of batch growth. Two pectin hydrolases and two pectin lyases by CM-Sepharose 6B, DEAE-cellulose, and Sephadex G-100 column chromatography were partially purified and characterized. Hydrolases I and II gave optimum activity at pH 5.5 and 4.5, respectively, at 50°C. For lyases I and II, the optimum occurred at pH 5.0 and 7.5, respectively, and 40°C.
Pectic enzymes from Aureobasidium pullulans LV10 / P.L. Manachini, C. Parini, M.G. Fortina. - In: ENZYME AND MICROBIAL TECHNOLOGY. - ISSN 0141-0229. - 10:11(1988), pp. 682-685. [10.1016/0141-0229(88)90060-9]
Pectic enzymes from Aureobasidium pullulans LV10
P.L. ManachiniPrimo
;C. PariniSecondo
;M.G. FortinaUltimo
1988
Abstract
• Aureobasidium pullulans LV 10 produced extracellular pectolytic activity when grown in a medium containing apple pectin as a carbon source. Maximum enzyme production (22 PGU ml−1 and 9 PLU ml−1) was obtained after 4 days of batch growth. Two pectin hydrolases and two pectin lyases by CM-Sepharose 6B, DEAE-cellulose, and Sephadex G-100 column chromatography were partially purified and characterized. Hydrolases I and II gave optimum activity at pH 5.5 and 4.5, respectively, at 50°C. For lyases I and II, the optimum occurred at pH 5.0 and 7.5, respectively, and 40°C.
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