Introduction of the Lactobacillus casei lactate dehydrogenase (LDH) gene into Saccharomyces cerevisiae under the control of the TPII promoter yielded high LDH levels in batch and chemostat cultures. LDH expression did not affect the dilution rate above which respiro-fermentative metabolism occurred (D-c.) in aerobic, glucose-limited chemostats. Above D-c, the LDH-expressing strain produced both ethanol and lactate, but its overall fermentation rate was the same as in wild-type cultures. Exposure of respiring, LDH-expressing cultures to glucose excess triggered simultaneous ethanol and lactate production. However, the specific glucose consumption rate was not affected, indicating that NADH reoxidation does not control glycolytic flux under these conditions. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
|Titolo:||NADH reoxidation does not control glycolytic flux during exposure of respiring Saccharomyces cerevisiae cultures to glucose excess|
|Parole Chiave:||Aerobic fermentation; Crabtree effect; Lactate dehydrogenase; NADH reoxidation; Yeast|
|Settore Scientifico Disciplinare:||Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni|
|Data di pubblicazione:||1999|
|Digital Object Identifier (DOI):||10.1016/S0378-1097(98)00589-8|
|Appare nelle tipologie:||01 - Articolo su periodico|