Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.
|Titolo:||Clinical utility of fractionating erythrocytes into "Percoll" density gradients|
MOSCA, ANDREA (Primo)
PALEARI, RENATA (Secondo)
|Parole Chiave:||Centrifugation, Density Gradient; Phytic Acid; Drug Compounding; Humans; Pyruvate Kinase; Erythrocytes; Anemia, Hemolytic; Glucosephosphate Dehydrogenase Deficiency; Child; Silicon Dioxide; Thalassemia; Cell Fractionation; Hexokinase; Fructose-Bisphosphate Aldolase; Clinical Enzyme Tests; Heterozygote; Glucosephosphate Dehydrogenase; Adult; Povidone; Erythrocyte Aging; Phosphogluconate Dehydrogenase; Reticulocytes; Male; Female|
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
|Data di pubblicazione:||1991|
|Appare nelle tipologie:||01 - Articolo su periodico|