Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.

Clinical utility of fractionating erythrocytes into "Percoll" density gradients / A. Mosca, R. Paleari, A. Modenese, S. Rossini, R. Parma, C. Rocco, V. Russo, G. Caramenti, M.L. Paderi, R. Galanello. - 307(1991), pp. 227-238.

Clinical utility of fractionating erythrocytes into "Percoll" density gradients

A. Mosca
Primo
;
R. Paleari
Secondo
;
V. Russo;
1991

Abstract

Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.
Centrifugation, Density Gradient; Phytic Acid; Drug Compounding; Humans; Pyruvate Kinase; Erythrocytes; Anemia, Hemolytic; Glucosephosphate Dehydrogenase Deficiency; Child; Silicon Dioxide; Thalassemia; Cell Fractionation; Hexokinase; Fructose-Bisphosphate Aldolase; Clinical Enzyme Tests; Heterozygote; Glucosephosphate Dehydrogenase; Adult; Povidone; Erythrocyte Aging; Phosphogluconate Dehydrogenase; Reticulocytes; Male; Female
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/193456
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 10
  • ???jsp.display-item.citation.isi??? ND
social impact