We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are simplicity, high reproducibility, no need for pre-treatment of samples, automatic correction of sample blanks, and speed and direct measurement of enzymatic reaction. The proposed methods are linear up to 7400 U/L at 30 degrees C and correlate well with the manual spectrophotometric method of Ellman for plasma cholinesterase and for washed erythrocytes. We adapted the same technique for the determination of erythrocyte cholinesterase using whole blood as sample and quinidine sulphate as inhibitor of pseudocholinesterase.

Measurement of erythrocyte acetylcholinesterase and plasma cholinesterase activity by a differential pH technique / L. Barenghi, F. Ceriotti, M. Luzzana, M. Ripamonti, A. Mosca, P. A. Bonini. - In: ANNALS OF CLINICAL BIOCHEMISTRY. - ISSN 0004-5632. - 23 ( Pt 5):5(1986 Sep), pp. 538-45-545.

Measurement of erythrocyte acetylcholinesterase and plasma cholinesterase activity by a differential pH technique

A. Mosca
Penultimo
;
1986-09

Abstract

We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are simplicity, high reproducibility, no need for pre-treatment of samples, automatic correction of sample blanks, and speed and direct measurement of enzymatic reaction. The proposed methods are linear up to 7400 U/L at 30 degrees C and correlate well with the manual spectrophotometric method of Ellman for plasma cholinesterase and for washed erythrocytes. We adapted the same technique for the determination of erythrocyte cholinesterase using whole blood as sample and quinidine sulphate as inhibitor of pseudocholinesterase.
Detergents; Cholinesterases; Acetylcholinesterase; Quinidine; Hydrogen-Ion Concentration; Humans; Buffers; Erythrocytes; Spectrophotometry, Ultraviolet; Colorimetry
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/192385
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