This is a new electrochemical method for determination of lipase activity in biological fluids, including serum, plasma, and duodenal juice. Advantages of turbidimetric methods--short reaction time, and small sample and reagent volumes--are combined with those of titrimetric methods: measurement of absolute activity (i.e., no standardization required), saturated substrate conditions, and direct measurement of reaction products. The proposed method is easy, inexpensive, and takes only 3 min. Precision is good: CV = 3.74% within day and 7.3% between days at the clinical-decision concentration, CV = 1.86% within day and 4.65% between days for above-normal lipase activities. The standard curve is linear up to 4500 U/L. Results (y) correlate well with those by turbidimetry (x): y = 0.9287x - 65.3 (r = 0.9719). Reference values are between 0 and 130 U/L.
|Titolo:||Measurement of lipase activity by a differential pH technique|
|Parole Chiave:||Reference Values; Humans; Hydrogen-Ion Concentration; Oils; Aged; Emulsions; Hydrolysis; Lipase; Plant Oils; Colipases; Cations; Bile Acids and Salts; Buffers; Nephelometry and Turbidimetry; Adult; Triolein; Middle Aged; Substrate Specificity; Male; Female|
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
|Data di pubblicazione:||feb-1985|
|Appare nelle tipologie:||01 - Articolo su periodico|