A restriction analysis of a single covalently closed circular plasmid isolated from Bacillus licheniformis strain MP3 was performed. A restriction map of the 6·44 kb plasmid is presented. The existence of genetic markers such as antibiotics and heavy metal ions resistance was investigated. The availability of two single restriction sites and the stability of this plasmid make it ideal for development as a cloning vector.
ISOLATION AND PRELIMINARY CHARACTERIZATION OF PLASMID IN BACILLUS-LICHENIFORMIS STRAIN MP3 / C. PARINI, M. FORTINA, M. ROGGIANI, P. MANACHINI. - In: LETTERS IN APPLIED MICROBIOLOGY. - ISSN 0266-8254. - 9:5(1989), pp. 169-171. [10.1111/j.1472-765X.1989.tb00316.x]
ISOLATION AND PRELIMINARY CHARACTERIZATION OF PLASMID IN BACILLUS-LICHENIFORMIS STRAIN MP3
C. PariniPrimo
;M. FortinaSecondo
;P. ManachiniUltimo
1989
Abstract
A restriction analysis of a single covalently closed circular plasmid isolated from Bacillus licheniformis strain MP3 was performed. A restriction map of the 6·44 kb plasmid is presented. The existence of genetic markers such as antibiotics and heavy metal ions resistance was investigated. The availability of two single restriction sites and the stability of this plasmid make it ideal for development as a cloning vector.
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