Two pectic lyases, L1 and L2, from culture liquids of Aureobasidium pullulans LV 10 were partially purified by ultrafiltration, CM-Sepharose 6B, DEAE-cellulose and/or Sephadex G 100 column chromatography, and characterized. L1 and L2 showed optimum activity at pH 5 and 7.5 respectively, and at 40°C. The molecular weights of the enzymes determined by gel filtration were estimated to be 89000 1000 and 55000 1000 for L1 and L2 respectively. Both lyases were activated by Ca2+ ions. L1 attacked highly esterified pectins, L2 attacked low methoxy-pectins in preference to polygalacturonic acid.
PROPERTIES OF 2 PECTIN LYASES PRODUCED BY AUREOBASIDIUM-PULLULANS LV-10 / C. PARINI, M. FORTINA, P. MANACHINI. - In: JOURNAL OF APPLIED BACTERIOLOGY. - ISSN 0021-8847. - 65:6(1988), pp. 477-481. [10.1111/j.1365-2672.1988.tb01920.x]
PROPERTIES OF 2 PECTIN LYASES PRODUCED BY AUREOBASIDIUM-PULLULANS LV-10
C. PariniPrimo
;M. FortinaSecondo
;P. ManachiniUltimo
1988
Abstract
Two pectic lyases, L1 and L2, from culture liquids of Aureobasidium pullulans LV 10 were partially purified by ultrafiltration, CM-Sepharose 6B, DEAE-cellulose and/or Sephadex G 100 column chromatography, and characterized. L1 and L2 showed optimum activity at pH 5 and 7.5 respectively, and at 40°C. The molecular weights of the enzymes determined by gel filtration were estimated to be 89000 1000 and 55000 1000 for L1 and L2 respectively. Both lyases were activated by Ca2+ ions. L1 attacked highly esterified pectins, L2 attacked low methoxy-pectins in preference to polygalacturonic acid.
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