The loss of bone due to trauma, infection, tumors and congenital deficiencies is still one of the most important issues in orthopaedic surgery and an innovative approach to solve this problem should include tissue engineering techniques. In order to determine the potential of ASCs to regenerate osteocondral defects in pre-clinical models, we focused our attention on the characterization and osteogenic differentiation of ASCs isolated from adipose tissue of rabbit (rbASCs) and pig (pASCs), which may be considered good pre-clinical models. We evaluated, in vitro, the ability of these cells to maintain their undifferentiated state and to differentiate into the osteogenic lineage when cultured in monolayer or in presence of a specific scaffold (hydroxyapatite). Our study suggests that autologous ASCs, alone or together with proper biomaterials, may be a suitable approach to repair critical bone defects in pre-clinical models

Autologous mesenchymal stem cells from adipose tissue (ASCs) in preclinical model : tissue engineering for bone regeneration / E. Arrigoni, S. Lopa, L. de Girolamo, D. Stanco, A.T. Brini1 - In: Proceeding of international conference on tissue engineering / [a cura di] P.J. Bartolo, P.R. Fernandes, R.B. Ruben, J. Folgado, H. Almeida, N. Alves, A. Mendes. - [s.l] : IST Press, 2009. - ISBN 972-978-8469-0-9. - pp. 73-79 (( Intervento presentato al 1. convegno International Conference on Tissue Engineering (ICTE) tenutosi a Leiria nel 2009.

Autologous mesenchymal stem cells from adipose tissue (ASCs) in preclinical model : tissue engineering for bone regeneration

E. Arrigoni
Primo
;
S. Lopa
Secondo
;
L. de Girolamo;D. Stanco
Penultimo
;
A.T. Brini1
Ultimo
2009

Abstract

The loss of bone due to trauma, infection, tumors and congenital deficiencies is still one of the most important issues in orthopaedic surgery and an innovative approach to solve this problem should include tissue engineering techniques. In order to determine the potential of ASCs to regenerate osteocondral defects in pre-clinical models, we focused our attention on the characterization and osteogenic differentiation of ASCs isolated from adipose tissue of rabbit (rbASCs) and pig (pASCs), which may be considered good pre-clinical models. We evaluated, in vitro, the ability of these cells to maintain their undifferentiated state and to differentiate into the osteogenic lineage when cultured in monolayer or in presence of a specific scaffold (hydroxyapatite). Our study suggests that autologous ASCs, alone or together with proper biomaterials, may be a suitable approach to repair critical bone defects in pre-clinical models
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/191941
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