In this paper we report an extensive NMR anal. of small ligands (Smac mimics) complexed with different constructs of XIAP. The mimics-binding site of XIAP is known as the BIR3 domain - primary, and the linker BIR2 region - secondary site. Interactions between the BIR3 domain and Smac mimics have been extensively studied by X-ray but, as of today, there are scarce data about the interaction between BIR2, or the whole linker-BIR2-BIR3 construct, and Smac mimics. In order to characterize our Smac mimics, we performed a STD NMR study between our 4-substituted, 1-aza-2-oxobicyclo[5.3.0]decane scaffold-based mols. and three different XIAP fragments: single BIR2 and BIR3 domains, and bifunctional linker-BIR2-BIR3. The results were integrated with docking calcns. and mol. dynamics simulations. NMR data, which are consistent with biol. tests, indicated that the two BIR subunits interact differently with our Smac mimics and suggest that the ligands enter into more intimate contact with the linker-BIR2-BIR3. In conclusion, we observe that the SMAC mimics showed with the construct linker-BIR2-BIR3 a series of NOE contacts that were not obsd. in the mono-domain ligand:BIR2 or :BIR3 complexes. So, in agreement with the computational models we believe that the linker moieties of the binding site play a key role in the stability of the protein complex.
A NMR and computational study of Smac mimics targeting both the BIR2 and BIR3 domains in XIAP protein / D. Potenza, L. Belvisi, F. Vasile, E. Moroni, F. Cossu, P. Seneci. - In: ORGANIC & BIOMOLECULAR CHEMISTRY. - ISSN 1477-0520. - 10:16(2012), pp. 3278-3287.
|Titolo:||A NMR and computational study of Smac mimics targeting both the BIR2 and BIR3 domains in XIAP protein|
POTENZA, DONATELLA (Primo)
BELVISI, LAURA (Secondo)
SENECI, PIERFAUSTO (Ultimo)
|Parole Chiave:||smac mimetic ; IAP proteins ; NMR ; computational chemistry ; apoptosis ; oncology|
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
Settore CHIM/01 - Chimica Analitica
Settore CHIM/08 - Chimica Farmaceutica
|Data di pubblicazione:||2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1039/c2ob06979b|
|Appare nelle tipologie:||01 - Articolo su periodico|