By means of optical and electron microscope autoradiography, following the administration of [3H]thymidine, we have detected a DNA replication near or on the nuclear membrane of meristematic cells of Haplopappus gracilis. This replication was observed only in the latter part of the S period. No localization to the nuclear membrane was seen at the beginning of the S period. Localization of the grains to the nuclear membrane in late S was observed both after short pulses and pulses as long as 1 h, and with chases of up to 2 h. It is concluded that the peripherally localized DNA replication is due to a DNA fraction located at the nuclear membrane, which replicates in the last part of the S period. It is suggested that such a DNA fraction may in part correspond to the constitutive heterochromatin.

Localization of DNA replicator sites near the nuclear membrane in plant cells / E. Sparvoli, M. G. Galli, A. Mosca, G. Paris. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 97:1(1976 Jan), pp. 74-82-82.

Localization of DNA replicator sites near the nuclear membrane in plant cells

A. Mosca;
1976

Abstract

By means of optical and electron microscope autoradiography, following the administration of [3H]thymidine, we have detected a DNA replication near or on the nuclear membrane of meristematic cells of Haplopappus gracilis. This replication was observed only in the latter part of the S period. No localization to the nuclear membrane was seen at the beginning of the S period. Localization of the grains to the nuclear membrane in late S was observed both after short pulses and pulses as long as 1 h, and with chases of up to 2 h. It is concluded that the peripherally localized DNA replication is due to a DNA fraction located at the nuclear membrane, which replicates in the last part of the S period. It is suggested that such a DNA fraction may in part correspond to the constitutive heterochromatin.
Chromosomes; Membranes; Floxuridine; Mitosis; Plants; DNA; Cell Nucleus; Cell Nucleolus; Autoradiography; DNA Replication
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/190411
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