Electrophoretic and isoelectric focusing separations have been achieved at -20 to -30°C, i.e., at temperatures considerably lower than previously reported by using as supporting media gels of acrylamide-methylacrylate copolymers and dimenthylsulfoxide-water mixtures. Hybrids of human and sickle cell hemoglobin and partially oxidized human carboxyhemoglobin have been separated in the temperature range -20 to -30°C, both by a discontinuous buffer gel electrophoresis and by isoelectric focusing.
Isoelectric focusing and electrophoresis at subzero temperatures / M. Perrella, A. Heyda, A. Mosca, L. Rossi-Bernardi. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - 88:1(1978 Jul 15), pp. 212-224.
Isoelectric focusing and electrophoresis at subzero temperatures
A. MoscaPenultimo
;
1978
Abstract
Electrophoretic and isoelectric focusing separations have been achieved at -20 to -30°C, i.e., at temperatures considerably lower than previously reported by using as supporting media gels of acrylamide-methylacrylate copolymers and dimenthylsulfoxide-water mixtures. Hybrids of human and sickle cell hemoglobin and partially oxidized human carboxyhemoglobin have been separated in the temperature range -20 to -30°C, both by a discontinuous buffer gel electrophoresis and by isoelectric focusing.
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