The inhibitory properties of procyanidins (a standardized oligomeric catechin fraction) from Vitis vinifera L. seeds on the respiratory burst and on the release of granule components myeloperoxidase, beta -glucuronidase and elastase were studied in activated human neutrophils. Procyanidins strongly inhibit superoxide generation with an IC50 of 7.2 muM, through a direct scavenging of superoxide and prevent the release from calcium ionophore activated neutrophils of beta -glucuronidase (IC50 = 13.9 muM), myeloperoxidase (IC50 = 7.2 muM) and elastase (IC50 = 5.4 muM). In addition they dose-dependently inhibit the activity of myeloperoxiclase released from calcium ionophore-stimulated cells with an IC50 value of 2 muM. The monomeric constitutive unit (+)-catechin was far less active than procyanidins in all the models tested. These results evidence that procyanidins efficiently restrain the inflammatory response of activated neutrophils in vitro and whenever absorbed in vivo can prevent their oxidative discharge at the site(s) of their adhesion.

Procyanidins from Vitis vinifera seeds inhibit the respiratory burst of activated human neutrophils and lysosomal enzyme release / M. Carini, R. Stefani, G. Aldini, M. Orioli, R. Maffei Facino. - In: PLANTA MEDICA. - ISSN 0032-0943. - 67:8(2001), pp. 714-717.

Procyanidins from Vitis vinifera seeds inhibit the respiratory burst of activated human neutrophils and lysosomal enzyme release

M. Carini
Primo
;
G. Aldini;M. Orioli
Penultimo
;
R. Maffei Facino
Ultimo
2001

Abstract

The inhibitory properties of procyanidins (a standardized oligomeric catechin fraction) from Vitis vinifera L. seeds on the respiratory burst and on the release of granule components myeloperoxidase, beta -glucuronidase and elastase were studied in activated human neutrophils. Procyanidins strongly inhibit superoxide generation with an IC50 of 7.2 muM, through a direct scavenging of superoxide and prevent the release from calcium ionophore activated neutrophils of beta -glucuronidase (IC50 = 13.9 muM), myeloperoxidase (IC50 = 7.2 muM) and elastase (IC50 = 5.4 muM). In addition they dose-dependently inhibit the activity of myeloperoxiclase released from calcium ionophore-stimulated cells with an IC50 value of 2 muM. The monomeric constitutive unit (+)-catechin was far less active than procyanidins in all the models tested. These results evidence that procyanidins efficiently restrain the inflammatory response of activated neutrophils in vitro and whenever absorbed in vivo can prevent their oxidative discharge at the site(s) of their adhesion.
Human neutrophils; Inhibitory effects; Procyanidins; Release of lysososmal enzymes; Respiratory burst; Vitaceae; Vitis vinifera L. seeds
Settore CHIM/08 - Chimica Farmaceutica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/187954
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 27
  • ???jsp.display-item.citation.isi??? ND
social impact