Protein phosphorylation in the brain represents a common target for several second messenger systems. A phosphoprotein (DARPP-32) specifically regulated by cAMP and dopamine has been detected in neurons bearing dopamine D-1 receptors, where it plays a key role in eliciting cAMP-mediated intracellular responses. The endogenous phosphorylation of this cytosolic protein is markedly affected after in vivo acute treatment with the selective D-1 agonist, SKF 38393. The amount of the DARPP-32 dephospho-form measured by a back-phosphorylation assay was decreased by about 30% in agonist-treated animals. This effect was completely counteracted by the concomitant administration of the selective D-1 antagonist, SCH 23390, but not by a selective D-2 antagonist. This first demonstration of in vivo modulation of the phosphorylation state of DARPP-32 could, as a biochemical approach, represent a useful tool to gain further insight into the cascade of biochemical events elicited by specific dopaminergic drugs.
IN VIVO MODULATION OF STRIATAL PHOSPHOPROTEINS BY DOPAMINERGIC AGENTS / M. DILUCA, M. CIMINO, M. ABBRACCHIO, F. CATTABENI. - In: EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION. - ISSN 0922-4106. - 172:4-5(1989), pp. 321-328.
|Titolo:||IN VIVO MODULATION OF STRIATAL PHOSPHOPROTEINS BY DOPAMINERGIC AGENTS|
DILUCA, MONICA MARIA GRAZIA (Primo)
ABBRACCHIO, MARIA PIA (Penultimo)
CATTABENI, FLAMINIO NICOLA (Ultimo)
|Parole Chiave:||(Pharmacological modulation); DARPP-32; Dopamine D-1 receptor; Protein phosphorylation; Striatum|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||1989|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0922-4106(89)90012-6|
|Appare nelle tipologie:||01 - Articolo su periodico|