Cultures preincubated in a growth restricted salts/glucose medium in the presence and absence of ornithine decarboxylase (ODC) activating factors were then incubated under ideal growth conditions to study the influence of these factors on cell growth. Incubation of confluent cultures in a salts/glucose medium alone did not induce ODC or change the other biochemical parameters investigated. However, if cultures were incubated in the salts/glucose medium supplemented with asparagine (ASN) and agents that increase cellular cAMP levels then ODC was induced after 6-8 h. This primary induction in the salts/glucose medium resulted in altered and delayed ODC induction during growth stimulation and also caused a delay in (3H) thymidine incorporation without affecting (3H) uridine and (3H) leucine incorporation. These results demonstrate that incubation of cultures in a salts/glucose media with ASN and dibutyryl cAMP (dBcAMP) causes refractory ODC induction and altered (3H) thymidine incorporation upon growth challenge with complete medium. These effects were not observed when cells were preincubated in a salts/glucose medium alone.

Cellular effects of ornithine decarboxylase induction in cells maintained with a salts/glucose medium / M. P. Abbracchio, M. Meloni, M. Costa. - In: LIFE SCIENCES. - ISSN 0024-3205. - 28:8(1981), pp. 937-944.

Cellular effects of ornithine decarboxylase induction in cells maintained with a salts/glucose medium

M. P. Abbracchio
Primo
;
1981

Abstract

Cultures preincubated in a growth restricted salts/glucose medium in the presence and absence of ornithine decarboxylase (ODC) activating factors were then incubated under ideal growth conditions to study the influence of these factors on cell growth. Incubation of confluent cultures in a salts/glucose medium alone did not induce ODC or change the other biochemical parameters investigated. However, if cultures were incubated in the salts/glucose medium supplemented with asparagine (ASN) and agents that increase cellular cAMP levels then ODC was induced after 6-8 h. This primary induction in the salts/glucose medium resulted in altered and delayed ODC induction during growth stimulation and also caused a delay in (3H) thymidine incorporation without affecting (3H) uridine and (3H) leucine incorporation. These results demonstrate that incubation of cultures in a salts/glucose media with ASN and dibutyryl cAMP (dBcAMP) causes refractory ODC induction and altered (3H) thymidine incorporation upon growth challenge with complete medium. These effects were not observed when cells were preincubated in a salts/glucose medium alone.
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/185546
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