Mobilized blood CD34+ cells from cancer patients were ex vivo infected by a recombinant adenovirus vector carrying an alkaline phosphatase gene, whose expression is evaluable by flow cytometry. A mean of 40% CD34+ cells were infected by the vector, with high levels of expression of the transgene. Among attempts to improve infection efficiency by manipulating culture conditions, only reinfection by the same vector achieved a 10% increase of transgene expression. Transduced CD34+ cells were induced to differentiate along the myeloid and the dendritic lineage, and in either case AP+ cells were detectable among the differentiated cell population. We conclude that adenovirus vectors may be useful tools for gene transduction into mobilized blood CD34+ cells, particularly for those applications in which high transgene expression for limited periods of time is required.
Adenovirus vectors for gene transduction into mobilized blood CD34+ cells / M. Bregni, S. Shammah, F. Malaffo, M. Di Nicola, M. Milanesi, M. Magni, P. Matteucci, F. Ravagnani, C. T. Jordan, S. Siena, A. M. Gianni. - In: GENE THERAPY. - ISSN 0969-7128. - 5:4(1998 Apr), pp. 465-472.
|Titolo:||Adenovirus vectors for gene transduction into mobilized blood CD34+ cells|
|Parole Chiave:||Adenoviridae; Dendritic Cells; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Transgenes; Gene Expression; Alkaline Phosphatase; Cell Differentiation; Lymphocytes; Histocytochemistry; Macrophage Colony-Stimulating Factor; Antigens, CD34; Polymerase Chain Reaction; Transfection; Cells, Cultured; Genetic Vectors; Flow Cytometry; Colony-Forming Units Assay; Time Factors|
|Settore Scientifico Disciplinare:||Settore MED/06 - Oncologia Medica|
|Data di pubblicazione:||apr-1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/sj.gt.3300620|
|Appare nelle tipologie:||01 - Articolo su periodico|