Somatic human cell hybrids produced by fusion of HeLa cells and diploid fibroblasts were analysed in a study designed to test the coordinate expression of transformation markers and tumorigenicity. The great majority of these hybrids displayed a finite lifespan in culture, but some of them inherited from the HeLa parent the capability to grow as permanent cell lines. Hybrids from both groups all had a plasminogen activator activity 20 to 100-fold higher and a cloning efficiency in semisolid medium 2 to 10-fold lower than the HeLa parent. Cell surface fibronectin was expressed at variable levels, albeit in a disorganized form. No correlation between the level of plasminogen activator or fibronectin content and cloning efficiency in agar was observed. Two hybrid lines, assayed for tumorigenicity in nude mice, did not produce tumors, even at inocula 20-fold greater than those at which the HeLa cells formed tumors.

Expression of transformation markers and suppression of tumorigenicity in human cell hybrids / L. Larizza, M. L. Tenchini, A. Mottura, L. De Carli, M. Colombi, S. Barlati. - In: EUROPEAN JOURNAL OF CANCER & CLINICAL ONCOLOGY. - ISSN 0277-5379. - 18:9(1982 Sep), pp. 845-851.

Expression of transformation markers and suppression of tumorigenicity in human cell hybrids

L. Larizza
Primo
;
M. L. Tenchini
Secondo
;
1982

Abstract

Somatic human cell hybrids produced by fusion of HeLa cells and diploid fibroblasts were analysed in a study designed to test the coordinate expression of transformation markers and tumorigenicity. The great majority of these hybrids displayed a finite lifespan in culture, but some of them inherited from the HeLa parent the capability to grow as permanent cell lines. Hybrids from both groups all had a plasminogen activator activity 20 to 100-fold higher and a cloning efficiency in semisolid medium 2 to 10-fold lower than the HeLa parent. Cell surface fibronectin was expressed at variable levels, albeit in a disorganized form. No correlation between the level of plasminogen activator or fibronectin content and cloning efficiency in agar was observed. Two hybrid lines, assayed for tumorigenicity in nude mice, did not produce tumors, even at inocula 20-fold greater than those at which the HeLa cells formed tumors.
Karyotyping; Animals; HeLa Cells; Humans; Genetic Markers; Mice; Mice, Nude; Neoplasms, Experimental; Fibroblasts; Neoplasm Transplantation; Oncogenes; Plasminogen Activators; Fibronectins; Hybrid Cells; Suppression, Genetic; Cell Transformation, Neoplastic; Cell Line
Settore MED/03 - Genetica Medica
Settore BIO/13 - Biologia Applicata
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/184352
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 12
  • ???jsp.display-item.citation.isi??? ND
social impact