An N-linked pyrene-dodecanoyl sulfatide was employed to measure the ratio of excimer fluorescence to monomer fluoresence intensities (E/M). The E/M values provided information about both the dynamic behavior and the structural distribution of the labelled glycolipid in note dispersion of micellar sulfatides and multilamellar vesicles of different phospholipids. Most of the labelled sulfatide seems to be located in domains sequestered from the surrounding phospholipids still above the phase transition temperature of the vesicles. The glycolipids sequestered in these domain environment are less sensitive to the structural changes that the addition of cholesterol or Ca2+ can induce in the phospholipid regions during the phase transition.

N-PYRENE DODECANOYL SULFATIDE AS MEMBRANE PROBE - A STUDY OF GLYCOLIPID DYNAMIC BEHAVIOR IN MODEL MEMBRANES / P. VIANI, C. GALIMBERTI, S. MARCHESINI, G. CERVATO, B. CESTARO. - In: CHEMISTRY AND PHYSICS OF LIPIDS. - ISSN 0009-3084. - 46:2(1988), pp. 89-97. [10.1016/0009-3084(88)90118-1]

N-PYRENE DODECANOYL SULFATIDE AS MEMBRANE PROBE - A STUDY OF GLYCOLIPID DYNAMIC BEHAVIOR IN MODEL MEMBRANES

P. Viani
Primo
;
B. Cestaro
Ultimo
1988

Abstract

An N-linked pyrene-dodecanoyl sulfatide was employed to measure the ratio of excimer fluorescence to monomer fluoresence intensities (E/M). The E/M values provided information about both the dynamic behavior and the structural distribution of the labelled glycolipid in note dispersion of micellar sulfatides and multilamellar vesicles of different phospholipids. Most of the labelled sulfatide seems to be located in domains sequestered from the surrounding phospholipids still above the phase transition temperature of the vesicles. The glycolipids sequestered in these domain environment are less sensitive to the structural changes that the addition of cholesterol or Ca2+ can induce in the phospholipid regions during the phase transition.
Settore BIO/10 - Biochimica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/184346
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 22
social impact