Using the N-hydroxysuccinimide ester of the fatty acids, galactosylceramide I3-sulfate containing a 5-or 16-doxyl-stearoyl residue was prepared in good yield by acylation of the galactosylsphingosine I3-sulfate (lysosulfatide) obtained from the saponification of the bovine brain sulfatide. The EPR behavior of the two semisynthetic sulfatides was analyzed in natural sulfatide micelles and in multilamellar vesicles of egg phosphatidylcholine. The evaluated parameters demonstrate that these spin-labelled sulfatides can be used for the study of sulfatide behavior in lipid structures.
PREPARATION OF SPIN-LABELED SULFATIDES FOR ELECTRON-PARAMAGNETIC-RES STUDIES ON MODEL MEMBRANES / S. MARCHESINI, P. VIANI, G. CERVATO, D. MONTI, B. CESTARO, C. OLIVA. - In: BIOCHEMISTRY INTERNATIONAL. - ISSN 0158-5231. - 14:3(1987), pp. 491-499.
PREPARATION OF SPIN-LABELED SULFATIDES FOR ELECTRON-PARAMAGNETIC-RES STUDIES ON MODEL MEMBRANES
P. VIANISecondo
;B. CESTAROPenultimo
;C. OLIVAUltimo
1987
Abstract
Using the N-hydroxysuccinimide ester of the fatty acids, galactosylceramide I3-sulfate containing a 5-or 16-doxyl-stearoyl residue was prepared in good yield by acylation of the galactosylsphingosine I3-sulfate (lysosulfatide) obtained from the saponification of the bovine brain sulfatide. The EPR behavior of the two semisynthetic sulfatides was analyzed in natural sulfatide micelles and in multilamellar vesicles of egg phosphatidylcholine. The evaluated parameters demonstrate that these spin-labelled sulfatides can be used for the study of sulfatide behavior in lipid structures.
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