Liquid chromatography/mass spectrometry (particle beam interface, electron impact ionization), fast-atom bombardment mass spectrometry (FAB-MS) (negative-ion mode) and FAB-tandem mass spectrometry (MS/MS) techniques were applied for the rapid detection/characterization of low-to-intermediate molecular weight polyphenol constituents of Krameria triandra roots with radical scavenging activity; this determined in a well established membrane model of lipid peroxidation (phosphatidylcholine liposomes). LC/MS analysis of the more lipophilic fractions of the drug (antioxidant activity: ICS,, the concentration which gives 50% inhibition, 5.8 mu g/mL petrol extract; IC50 3.7 mu g/mL chloroform extract) evidences at least 9 components belonging to the class of lignans; FAB-MS/MS analysis of the butylacetate extract (IC50 = 0.23 mu g/mL) caffeoyl esters, catechin-glucosides, dimeric procyanidines and their corresponding phenylpropanoyd derivatives.

A rapid screening by liquid chromatography mass spectrometry and fast-atom bombardment tandem mass spectrometry of phenolic constituents with radical scavenging activity, from Krameria triandra roots / R. Maffei Facino, M. Carini, G. Aldini, L. De Angelis. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - 11:12(1997), pp. 303-308.

A rapid screening by liquid chromatography mass spectrometry and fast-atom bombardment tandem mass spectrometry of phenolic constituents with radical scavenging activity, from Krameria triandra roots

R. Maffei Facino
Primo
;
M. Carini
Secondo
;
G. Aldini
Penultimo
;
1997

Abstract

Liquid chromatography/mass spectrometry (particle beam interface, electron impact ionization), fast-atom bombardment mass spectrometry (FAB-MS) (negative-ion mode) and FAB-tandem mass spectrometry (MS/MS) techniques were applied for the rapid detection/characterization of low-to-intermediate molecular weight polyphenol constituents of Krameria triandra roots with radical scavenging activity; this determined in a well established membrane model of lipid peroxidation (phosphatidylcholine liposomes). LC/MS analysis of the more lipophilic fractions of the drug (antioxidant activity: ICS,, the concentration which gives 50% inhibition, 5.8 mu g/mL petrol extract; IC50 3.7 mu g/mL chloroform extract) evidences at least 9 components belonging to the class of lignans; FAB-MS/MS analysis of the butylacetate extract (IC50 = 0.23 mu g/mL) caffeoyl esters, catechin-glucosides, dimeric procyanidines and their corresponding phenylpropanoyd derivatives.
Settore CHIM/08 - Chimica Farmaceutica
1997
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/183942
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