Bovine neutrophils (PMNs) activated by incubation of blood samples in presence of lipopolysaccharides (LPS) showed changes in density levels respect to non activated samples, as previously observed under spontaneous inflammatory conditions. By the mean of discontinuous Percoll gradients it was possible to separate blood PMNs with very high yield and purity percentages at the highest Percoll concentration used. In activated samples the percentage of purity affects that of recover. The latter appears improved if the recover includes not only the cells that settle in correspondence of the highest Percoll concentration, but also those at the mean Percoll concentration, thus only slightly compromising purity levels. Flow cytometry demonstrated that activated PMNs are larger and show lower density and higher expression of integrins CD11b than non activated PMNs.

Attivazione in vitro di granulociti neutrofili di bovino: modificazione della densità e dell’espressione di integrine / V. Spagnolo, A. Giordano, S. Comazzi, P. Sartorelli - In: Atti I Congresso Nazionale AIPVet[s.l] : Associazione Italiana Patologi Veterinari, 2004. (( convegno I Congresso Nazionale AIPVet tenutosi a Gargnano (BS).

Attivazione in vitro di granulociti neutrofili di bovino: modificazione della densità e dell’espressione di integrine

A. Giordano
Secondo
;
S. Comazzi
Penultimo
;
P. Sartorelli
Ultimo
2004

Abstract

Bovine neutrophils (PMNs) activated by incubation of blood samples in presence of lipopolysaccharides (LPS) showed changes in density levels respect to non activated samples, as previously observed under spontaneous inflammatory conditions. By the mean of discontinuous Percoll gradients it was possible to separate blood PMNs with very high yield and purity percentages at the highest Percoll concentration used. In activated samples the percentage of purity affects that of recover. The latter appears improved if the recover includes not only the cells that settle in correspondence of the highest Percoll concentration, but also those at the mean Percoll concentration, thus only slightly compromising purity levels. Flow cytometry demonstrated that activated PMNs are larger and show lower density and higher expression of integrins CD11b than non activated PMNs.
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
http://www.aipvet.it/APIVMeetings/2004_Atti_AIPVet/Documenti/Atti_AIPVET_2004.pdf
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/183630
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