As an alternative to the recently proposed screening for β-thalassemias by isoelectric focusing (IEF) of denatured globin chains in urea-detergent gels , IEF of intact hemoglobins, obtained from umbilical cord blood in neonatal units, is reported here. For maximum separation, IEF should be performed in nonlinear pH gradients, in gels containing 0.2 M β-alanine and 0.2 M 6-amino caproic acid, which flatten the pH gradient around pH 7, thus increasing the resolution between HbA and HbF(ac). The method can unambiguously detect homozygous and heterozygous β-thalassemic conditions. A bimodal distribution of HbA at birth has been found: In heterozygous patients, HbA values of 9.02% (range 6.8-9.98%) have been found while in normal newborns HbA levels of 20.34% (range 11.02-30.6%) have been demonstrated.
|Titolo:||Neonatal screening of beta-thalassemias by thin-layer isoelectric focusing|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
|Data di pubblicazione:||1982|
|Digital Object Identifier (DOI):||10.1002/ajh.2830130207|
|Appare nelle tipologie:||01 - Articolo su periodico|