Slot-blot hybridization technique was used to evaluate growth hormone-releasing hormone (GHRH) mRNA levels in the hypothalamus of long-term (14 days) hypophysectomized (HPX) rats treated or not with 125 micrograms hGH/rat, twice daily IP, since the first day postsurgery. In addition, mRNA levels were determined in the hypothalamus of short-term (4 days) GH-treated (250 micrograms hGH/rat, twice daily IP) intact rats. GHRH mRNA levels were increased in HPX rats, and GH treatment partially counteracted this rise. Short-term administration of GH decreased GHRH mRNA levels in intact rats. These results, evaluated together with previous findings showing decreased hypothalamic GHRH-like immunoreactivity in both HPX rats and intact rats given GH (6, 7, 9), indicate that GH exerts a negative feedback action on the synthesis and release of GHRH.
Growth hormone regulation of growth hormone-releasing hormone gene expression / V. De GENNARO COLONNA, E. CATTANEO, D. COCCHI, E. MULLER, A. MAGGI. - In: PEPTIDES. - ISSN 0196-9781. - 9:5(1988 Oct), pp. 985-988.
Titolo: | Growth hormone regulation of growth hormone-releasing hormone gene expression |
Autori: | DE GENNARO COLONNA, VITO (Primo) CATTANEO, ELENA (Secondo) MULLER, EUGENIO (Penultimo) MAGGI, ADRIANA CATERINA (Ultimo) |
Settore Scientifico Disciplinare: | Settore BIO/14 - Farmacologia |
Data di pubblicazione: | ott-1988 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0196-9781(88)90077-0 |
Appare nelle tipologie: | 01 - Articolo su periodico |