Slot-blot hybridization technique was used to evaluate growth hormone-releasing hormone (GHRH) mRNA levels in the hypothalamus of long-term (14 days) hypophysectomized (HPX) rats treated or not with 125 micrograms hGH/rat, twice daily IP, since the first day postsurgery. In addition, mRNA levels were determined in the hypothalamus of short-term (4 days) GH-treated (250 micrograms hGH/rat, twice daily IP) intact rats. GHRH mRNA levels were increased in HPX rats, and GH treatment partially counteracted this rise. Short-term administration of GH decreased GHRH mRNA levels in intact rats. These results, evaluated together with previous findings showing decreased hypothalamic GHRH-like immunoreactivity in both HPX rats and intact rats given GH (6, 7, 9), indicate that GH exerts a negative feedback action on the synthesis and release of GHRH.
Growth hormone regulation of growth hormone-releasing hormone gene expression / V. De GENNARO COLONNA, E. CATTANEO, D. COCCHI, E. MULLER, A. MAGGI. - In: PEPTIDES. - ISSN 0196-9781. - 9:5(1988 Oct), pp. 985-988.
|Titolo:||Growth hormone regulation of growth hormone-releasing hormone gene expression|
DE GENNARO COLONNA, VITO (Primo)
CATTANEO, ELENA (Secondo)
MULLER, EUGENIO (Penultimo)
MAGGI, ADRIANA CATERINA (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||ott-1988|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0196-9781(88)90077-0|
|Appare nelle tipologie:||01 - Articolo su periodico|