Tramadol quant. detn. by gas chromatog.-mass spectrometry (GC/MS) using nefopam hydrochloride as internal std. (IS) and two calibration curves because of the large range of concn. attended in the plasmatic samples is described. Plasma samples drawn from subjects in postoperative period treated with two different initial i.v. bolus of tramadol (50 and 100 mg) followed by tramadol at the same infusion rate (12 mg h-1) are analyzed. We operated for the qual. anal. in Scan mode while for the quant. anal. in SIM mode, selecting the ion m/z 58 for tramadol and m/z 179 for IS. The limit of detection (LOD) was 0.01 μg ml-1 and the limit of quantification was 0.04 μg ml-1.

Validation of a GC/MS method for the determination of tramadol in human plasma after intravenous bolus / V. Gambaro, C. Benvenuti, L. D. Ferrari, L. Dell'Acqua, F. Farè. - In: IL FARMACO. - ISSN 0014-827X. - 58:9(2003), pp. 947-950. [10.1016/S0014-827X(03)00153-8]

Validation of a GC/MS method for the determination of tramadol in human plasma after intravenous bolus

V. Gambaro
Primo
;
L. Dell'Acqua
Penultimo
;
F. Farè
Ultimo
2003

Abstract

Tramadol quant. detn. by gas chromatog.-mass spectrometry (GC/MS) using nefopam hydrochloride as internal std. (IS) and two calibration curves because of the large range of concn. attended in the plasmatic samples is described. Plasma samples drawn from subjects in postoperative period treated with two different initial i.v. bolus of tramadol (50 and 100 mg) followed by tramadol at the same infusion rate (12 mg h-1) are analyzed. We operated for the qual. anal. in Scan mode while for the quant. anal. in SIM mode, selecting the ion m/z 58 for tramadol and m/z 179 for IS. The limit of detection (LOD) was 0.01 μg ml-1 and the limit of quantification was 0.04 μg ml-1.
GC/MS; Tramadol; Validation
Settore CHIM/08 - Chimica Farmaceutica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/182863
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