Changes in the concentration of major serum proteins were monitored from day 0 to day 4 in three experimental groups: rats injected with turpentine, rats receiving the turpentine shot and daily doses of indomethacine, and rats given indomethacine alone. In inflamed animals, peak changes for acute-phase reactants, evaluated by two-dimensional electrophoresis (2-DE), were usually observed between 48 and 72 h after the phlogistic stimulus. By itself, indomethacine was found to affect the synthesis of most proteins (except one of the thiostatin variants and ceruloplasmin); the changes in serum levels, whether positive or negative, were the same as upon inflammation (except for kallikrein-binding protein), but their extent and/or timing usually differed. When inflamed animals were given indomethacine, a clear-cut difference in the concentration of some proteins was observed versus inflamed rats not given medication, at 24 h after the start of the treatments. Proteins mainly affected were α2-macroglobulin, α2-HS-glycoprotein, C-reactive protein and kallikrein-binding protein.
Proteins of rat serum IV. Time-course of acute-phase protein expression and its modulation by indomethacine / I. Eberini, I. Miller, V. Zancan, C. Bolego, L. Puglisi, M. Gemeiner, E. Gianazza. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 20:4-5(1999), pp. 846-853.
|Titolo:||Proteins of rat serum IV. Time-course of acute-phase protein expression and its modulation by indomethacine|
EBERINI, IVANO (Primo)
GIANAZZA, ELISABETTA (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
Settore BIO/14 - Farmacologia
|Data di pubblicazione:||1999|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/(SICI)1522-2683(19990101)20:4/5<846::AID-ELPS846>3.0.CO;2-2|
|Appare nelle tipologie:||01 - Articolo su periodico|