By performing electrphoresis perpendicular to a stationary pH gradient generated by focused carrier ampholytes in a gel slab, a pictorial representation of a protein titration curve is obtained. By running a protein and its genetic mutants in parallel, the shape of the relative titration curve reveals which charged amino acid has been substituted. Within a given family of proteins, under a constant set of experimental conditions, the relative mobility at any given pH can be correlated with the number of protons lost or acquired by the protein.
Protein titration curves by combined isoelectric focusing-electrophoresis with hemoglobin mutants as models / P.G. Righetti, R. Krishnamoorthy, E. Gianazza, D. Labie. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 166:2(1978), pp. 455-460.
Protein titration curves by combined isoelectric focusing-electrophoresis with hemoglobin mutants as models
E. GianazzaPenultimo
;
1978
Abstract
By performing electrphoresis perpendicular to a stationary pH gradient generated by focused carrier ampholytes in a gel slab, a pictorial representation of a protein titration curve is obtained. By running a protein and its genetic mutants in parallel, the shape of the relative titration curve reveals which charged amino acid has been substituted. Within a given family of proteins, under a constant set of experimental conditions, the relative mobility at any given pH can be correlated with the number of protons lost or acquired by the protein.
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