Two mechanisms for the cathodic drift in isoelectric focusing are proposed: (1) Ampholine-Ampholine interaction between acidic and basic species, particularly in the pH range 6-7; the aggregates are slowly split during the focusing process, to an extent proportional to the square of the voltage applied; and (2) the presence of transient species of "poor" carrier ampholytes, particularly at allkaline pH. The resultant of these two "transient states" is to set in motion a "cascade" effect on the "steady-state" species, the net effect of which is a drift to the cathode. The markedly different behaviour of acidic and basic carrier ampholytes is demostrated via binding to a neutral detergent, Nonidet P40.

Ampholine-ampholine interaction as a couse of pH gradient drift in isoelectric focusing / E. Gianazza, C. Astorri, P.G. Righetti. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 171:C(1979), pp. 161-169. [10.1016/S0021-9673(01)95296-3]

Ampholine-ampholine interaction as a couse of pH gradient drift in isoelectric focusing

E. Gianazza
Primo
;
1979

Abstract

Two mechanisms for the cathodic drift in isoelectric focusing are proposed: (1) Ampholine-Ampholine interaction between acidic and basic species, particularly in the pH range 6-7; the aggregates are slowly split during the focusing process, to an extent proportional to the square of the voltage applied; and (2) the presence of transient species of "poor" carrier ampholytes, particularly at allkaline pH. The resultant of these two "transient states" is to set in motion a "cascade" effect on the "steady-state" species, the net effect of which is a drift to the cathode. The markedly different behaviour of acidic and basic carrier ampholytes is demostrated via binding to a neutral detergent, Nonidet P40.
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/182529
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