Two mechanisms for the cathodic drift in isoelectric focusing are proposed: (1) Ampholine-Ampholine interaction between acidic and basic species, particularly in the pH range 6-7; the aggregates are slowly split during the focusing process, to an extent proportional to the square of the voltage applied; and (2) the presence of transient species of "poor" carrier ampholytes, particularly at allkaline pH. The resultant of these two "transient states" is to set in motion a "cascade" effect on the "steady-state" species, the net effect of which is a drift to the cathode. The markedly different behaviour of acidic and basic carrier ampholytes is demostrated via binding to a neutral detergent, Nonidet P40.
|Titolo:||Ampholine-ampholine interaction as a couse of pH gradient drift in isoelectric focusing|
GIANAZZA, ELISABETTA (Primo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1979|
|Digital Object Identifier (DOI):||10.1016/S0021-9673(01)95296-3|
|Appare nelle tipologie:||01 - Articolo su periodico|