We report the statistical distributions of size and charge in protein molecules in living systems. The data have been plotted from more than 500 entries. 57% of the native proteins have molecular weights between 25,000 and 150,000 daltons. In oligomeric proteins, 71% of the subunits have molecular weights in the range 10,000-60,000. As for the subunit stoichiometry in oligomeric proteins, 50% of all the possible structures are dimeric, 30% tetrameric and 8% hexameric. More than one third of the proteins (38%) have pl values within 1.5 pH unit (pH4.5-6.0). 70% of the total proteins have pI values below pH 7 and 30% above it. On the basis of these data, guidelines are given for a purification strategy when using SDS-electrophoresis and isoelectric focusing.

Size and charge distribution of macromolecules in living systems / E. Gianazza, P.G. Righetti. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 193:1(1980), pp. 1-8.

Size and charge distribution of macromolecules in living systems

E. Gianazza
Primo
;
1980

Abstract

We report the statistical distributions of size and charge in protein molecules in living systems. The data have been plotted from more than 500 entries. 57% of the native proteins have molecular weights between 25,000 and 150,000 daltons. In oligomeric proteins, 71% of the subunits have molecular weights in the range 10,000-60,000. As for the subunit stoichiometry in oligomeric proteins, 50% of all the possible structures are dimeric, 30% tetrameric and 8% hexameric. More than one third of the proteins (38%) have pl values within 1.5 pH unit (pH4.5-6.0). 70% of the total proteins have pI values below pH 7 and 30% above it. On the basis of these data, guidelines are given for a purification strategy when using SDS-electrophoresis and isoelectric focusing.
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/182518
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