Isoelectric focusing studies of human pancreatic secretion

Pure bile, pancreatic and duodenal human juices have been analyzed by isoelectric focusing, either at rest or upon stimulation with caerulein. In rats, stimulation has also been performed by secretin. Twenty bands have been resolved and quantified in the pancreatic secretion. By developing zymograms, a number of isozymes have been identified: 6 iso-amylases [pl's 7.2, 7.1 and 6.6 (major) and pl's 7.4, 6.7 and 5.8 (minor)], 3 lipases [pl's 7.0 and 6.8 (major) and 6.4 (minor)], two major alkaline proteases (pl's 9.8 and 8.4) and one major acidic protease (pl 4.3) and one band of RNAase activity (pl 8.6). The stimulation kinetics follow a mechanism according to Palade, indicating uniform response to secretogogues, parallel intracellular transport and parallel discharge of pancreatic exocrine proteins.