An assay of high sensitivity and specificity for detection of residual malignant cells in remission bone marrow from patients with acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL) has been developed. The assay combines an immunoselection step with immunoglobulin gene rearrangement analysis by high specific-activity DNA probes. In experimental conditions, less than one contaminating tumor cell out of 1000 bone marrow cells was detected. A bone marrow contamination was detected in morphologically negative bone marrow specimens from two intermediate-grade non-Hodgkin's lymphoma patients. This method can be of value in determining a true remission status in high-risk ALL and NHL patients.

High sensitivity and specificity assay for detection of leukemia/lymphoma cells in human bone marrow / M. Bregni, S. Siena, R. Dalla-Favera, A. M. Gianni. - In: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES. - ISSN 0077-8923. - 511:(1987), pp. 473-82-482. [10.1111/j.1749-6632.1987.tb36279.x]

High sensitivity and specificity assay for detection of leukemia/lymphoma cells in human bone marrow

S. Siena;A. M. Gianni
1987

Abstract

An assay of high sensitivity and specificity for detection of residual malignant cells in remission bone marrow from patients with acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL) has been developed. The assay combines an immunoselection step with immunoglobulin gene rearrangement analysis by high specific-activity DNA probes. In experimental conditions, less than one contaminating tumor cell out of 1000 bone marrow cells was detected. A bone marrow contamination was detected in morphologically negative bone marrow specimens from two intermediate-grade non-Hodgkin's lymphoma patients. This method can be of value in determining a true remission status in high-risk ALL and NHL patients.
Leukemia, Lymphoid; Neoplastic Stem Cells; Genes, Immunoglobulin; Cell Separation; Nucleic Acid Hybridization; Bone Marrow Examination; DNA, Neoplasm; Antibodies, Monoclonal; Bone Marrow; Tumor Cells, Cultured; Burkitt Lymphoma; Flow Cytometry; Immunoglobulin Heavy Chains; Lymphoma, Non-Hodgkin
Settore MED/06 - Oncologia Medica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/181651
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