12-O-tetradecanoylphorbol-13-acetate (TPA) caused a rapid activation of protein kinase C in a murine (HEL-30) and in a human (NCTC) epidermal cell line. In HEL-30 cells, protein kinase C activation is followed by ornithine decarboxylase stimulation and cell proliferation, events inhibited by H-7, a specific inhibitor of protein kinase C. TPA in NCTC cells inhibited the basal ornithine decarboxylase activity and cell growth, whereas H-7 did not modify TPA effect. The response of NCTC cells was not due to direct toxicity of TPA. These data confirm that in murine epidermal cells, the proliferation induced by TPA is mediated by protein kinase C, whereas in a human skin-derived cell line these events are not or inversely associated.

Different effects of TPA on two skin-derived cell lines: Murine (HEL-30) and human (NCTC) epidermal cells / E. Corsini, P. Restani, C.L. Galli, M. Marinovich. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 191:1(1990), pp. 129-132.

Different effects of TPA on two skin-derived cell lines: Murine (HEL-30) and human (NCTC) epidermal cells

E. Corsini
Primo
;
P. Restani
Secondo
;
C.L. Galli
Penultimo
;
M. Marinovich
Ultimo
1990

Abstract

12-O-tetradecanoylphorbol-13-acetate (TPA) caused a rapid activation of protein kinase C in a murine (HEL-30) and in a human (NCTC) epidermal cell line. In HEL-30 cells, protein kinase C activation is followed by ornithine decarboxylase stimulation and cell proliferation, events inhibited by H-7, a specific inhibitor of protein kinase C. TPA in NCTC cells inhibited the basal ornithine decarboxylase activity and cell growth, whereas H-7 did not modify TPA effect. The response of NCTC cells was not due to direct toxicity of TPA. These data confirm that in murine epidermal cells, the proliferation induced by TPA is mediated by protein kinase C, whereas in a human skin-derived cell line these events are not or inversely associated.
Settore BIO/14 - Farmacologia
http://www.ncbi.nlm.nih.gov/pubmed/2226643
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/181077
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