Aldose reductase has been shown to be expressed in large amount by human embryonic epithelial cells (EUE) in response to osmotic stress. This conclusion is the result of studies undertaken following the purification to homogeneity of two forms of a 35-kDa protein overexpressed in EUE cells grown in hypertonic saline culture medium as compared to EUE cells grown in isoosmotic medium. Amino-acid composition, molecular weight and partial internal amino-acid sequence showed that the above proteins are two different forms of aldose reductase. These findings were confirmed by the observation that aldose reductase activity increased about 150-fold in adapted cells and returned to basal levels in de-adapted cells.
Aldose reductase is involved in long term adaptation of EUE cells to hyperosmotic stress / A. Ferraretto, A. Negri, A. Giuliani, L. De Grada, A.M. Fuhrman Conti, S. Ronchi. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - 1175:3(1993), pp. 283-288.
|Titolo:||Aldose reductase is involved in long term adaptation of EUE cells to hyperosmotic stress|
FERRARETTO, ANITA (Primo)
NEGRI, ARMANDO (Secondo)
RONCHI, SEVERINO (Ultimo)
|Parole Chiave:||(EUE cell); Aldose reductase; Cell adaptation; Hypertonic stress|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1993|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0167-4889(93)90218-E|
|Appare nelle tipologie:||01 - Articolo su periodico|