In this paper we refer a case of misidentification of medicinal plants involving dried petals of Papaver rhoeas contaminated with Papaver bracteatum petals. Preliminarily, TLC analysis evidenced the presence of thebaine either in the fluid extracts or in the petals. In this frame it was necessary to carry out an accurate botanic examination of the vegetable material which revealed the contamination of the red poppy petals with scarlet poppy petals. Moreover to confirm the adulteration, we developed and validated an efficient reverse phase ion pair HPLC method for the determination of the alkaloids contained in the Papaver species. Six petal batches and five commercial fluid extracts were analysed. Only one petal batch coming from Iran resulted to contain thebaine and its analogue oripavine, while all the fluid extracts contained the alkaloids typical of the Papaver bracteatum species, meaning that they were all prepared with contaminated petals.

Analysis of fluid extracts obtained with Papaver rhoeas petals contaminated with Papaver bracteatum petals / V. Gambaro, P. Minghetti, S. Arnoldi, M.L. Colombo, L. Dell’Acqua, A. Casiraghi, K. Guerrini, F. Farè, G. Roda. - In: PLANTA MEDICA. - ISSN 0032-0943. - 78:12(2012), pp. 1395-1398. [Epub ahead of print] [10.1055/s-0032-1314981]

Analysis of fluid extracts obtained with Papaver rhoeas petals contaminated with Papaver bracteatum petals

V. Gambaro
Primo
;
P. Minghetti
Secondo
;
S. Arnoldi;L. Dell’Acqua;A. Casiraghi;K. Guerrini;F. Farè
Penultimo
;
G. Roda
Ultimo
2012

Abstract

In this paper we refer a case of misidentification of medicinal plants involving dried petals of Papaver rhoeas contaminated with Papaver bracteatum petals. Preliminarily, TLC analysis evidenced the presence of thebaine either in the fluid extracts or in the petals. In this frame it was necessary to carry out an accurate botanic examination of the vegetable material which revealed the contamination of the red poppy petals with scarlet poppy petals. Moreover to confirm the adulteration, we developed and validated an efficient reverse phase ion pair HPLC method for the determination of the alkaloids contained in the Papaver species. Six petal batches and five commercial fluid extracts were analysed. Only one petal batch coming from Iran resulted to contain thebaine and its analogue oripavine, while all the fluid extracts contained the alkaloids typical of the Papaver bracteatum species, meaning that they were all prepared with contaminated petals.
ion pair reversed-phase HPLC; oripavine; Papaver bracteatum; Papaver rhoeas; Papaveraceae; thebaine
Settore CHIM/08 - Chimica Farmaceutica
Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
2012
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/180679
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