Among Triticum species two different Au and Am genomes derived from Triticum urartu and Triticum monococcum, respectively, were recognized. Au and Am genomes share similar chromosomal organization, notwithstanding, significant molecular divergences have been assessed using sequence data and AFLP markers, supporting chimeric Au /Am chromosomes as a tool to dissect their genetic differences. Interspecific introgression line (IL) libraries have been used extensively in plants as their power to detect QTLs with small effects is higher compared to mapping populations having whole genome fragments segregating. In addition, interspecific ILs are a valuable tool for adding foreign DNA in "elite" varieties to improve their agronomic traits. Sterility as well as low levels of viability can affect the development of intespecific ILs despite that, they were successfully obtained for tomato, watermelon and barley. Offsprings obtained from crosses between T. urartu and T. monococcum were reported to be sterile, but rare fertile F1 plants were obtained by crossing the "elite" einkorn accession L118 with T. urartu ID388. From these fertile genotypes, ILs were developed backcrossing F1 hybrid lines for several times with T. monococcum L118 as recurrent parent. Hundreds of mapped codominant markers in einkorn are currently used to characterize the exotic DNA fragment in each IL and to construct a panel of ILs carrying overlapping chromosome fragments of T. urartu. As T. monococcum L118 and T. urartu ID388 differ in the grain content of Zn, Ca, carotenoids, tocols as well as in other important agronomic traits, the panel of ILs will be a valuable tool to gain insights on the key loci controlling micronutrient content of kernel in diploid wheats. The phenotyping of the IL panel for some important traits associated with micronutrient content in kernel as well as statistical analyses to identify contingent or transgressive QTLs are currently underway.
Towards the construction of an interspecific introgression line library in einkorn using Triticum urartu / A. Fricano, A. Brandolini, D. Boyer, A. Hidalgo, D. Erba, P. Sourdille, F. Salamini, P. Piffanelli - In: 2 International Symposium on Genomics of Plant Genetic Resources / [a cura di] E. Frison, A. Graner, R. Tuberosa. - [s.l] : Università di Bologna, 2010. - pp. 182-182 (( Intervento presentato al 2. convegno International Symposium on Genomics of Plant Genetic Resources tenutosi a Bologna nel 2010.
Towards the construction of an interspecific introgression line library in einkorn using Triticum urartu
A. Hidalgo;D. Erba;
2010
Abstract
Among Triticum species two different Au and Am genomes derived from Triticum urartu and Triticum monococcum, respectively, were recognized. Au and Am genomes share similar chromosomal organization, notwithstanding, significant molecular divergences have been assessed using sequence data and AFLP markers, supporting chimeric Au /Am chromosomes as a tool to dissect their genetic differences. Interspecific introgression line (IL) libraries have been used extensively in plants as their power to detect QTLs with small effects is higher compared to mapping populations having whole genome fragments segregating. In addition, interspecific ILs are a valuable tool for adding foreign DNA in "elite" varieties to improve their agronomic traits. Sterility as well as low levels of viability can affect the development of intespecific ILs despite that, they were successfully obtained for tomato, watermelon and barley. Offsprings obtained from crosses between T. urartu and T. monococcum were reported to be sterile, but rare fertile F1 plants were obtained by crossing the "elite" einkorn accession L118 with T. urartu ID388. From these fertile genotypes, ILs were developed backcrossing F1 hybrid lines for several times with T. monococcum L118 as recurrent parent. Hundreds of mapped codominant markers in einkorn are currently used to characterize the exotic DNA fragment in each IL and to construct a panel of ILs carrying overlapping chromosome fragments of T. urartu. As T. monococcum L118 and T. urartu ID388 differ in the grain content of Zn, Ca, carotenoids, tocols as well as in other important agronomic traits, the panel of ILs will be a valuable tool to gain insights on the key loci controlling micronutrient content of kernel in diploid wheats. The phenotyping of the IL panel for some important traits associated with micronutrient content in kernel as well as statistical analyses to identify contingent or transgressive QTLs are currently underway.
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