Enrichment cultures on naphtha solvent were used to select aromatic hydrocarbon-degrading bacteria from a BTEX (benzene, toluene, ethylbenzene, xylene)-contaminated subsoil obtained from beneath a paint factory located in Milan, Italy. Fifteen isolated strains were studied for their different biodegradative capacities. Among these, 13 were able to grow on naphtha solvent. Ten were identified as Pseudomonas putida and three as Pseudomonas aureofaciens. Two other degraders were identified as Pseudomonas aeruginosa and Alcaligenes xylosoxidans subsp, denitrificans. Further molecular characterization of the isolates was carried out by randomly amplified polymorphic DNA analysis to ascertain that all the studied strains belonged to different haplotypes. The isolates were characterized for the presence of genes encoding for toluene dioxygenase, xylene monooxygenase and catechol 2,3-dioxygenase by polymerase chain reaction analysis and by Southern analysis. P, putida strain CM23, which showed homology with xylA,M, xylE and todC1C2BA genes, possessed multiple pathways which enabled the strain to grow on benzene, toluene and m-xylene.

Distribution of catabolic pathways in some hydrocarbon-degrading bacteria from a subsurface polluted soil / L. Cavalca, P. Di Gennaro, M. Colombo, V. Andreoni, S. Bernasconi, I. Ronco, G. Bestetti. - In: RESEARCH IN MICROBIOLOGY. - ISSN 0923-2508. - 151:10(2000), pp. 877-887.

Distribution of catabolic pathways in some hydrocarbon-degrading bacteria from a subsurface polluted soil

L. Cavalca
Primo
;
P. Di Gennaro
Secondo
;
M. Colombo;V. Andreoni;S. Bernasconi;I. Ronco
Penultimo
;
G. Bestetti
Ultimo
2000

Abstract

Enrichment cultures on naphtha solvent were used to select aromatic hydrocarbon-degrading bacteria from a BTEX (benzene, toluene, ethylbenzene, xylene)-contaminated subsoil obtained from beneath a paint factory located in Milan, Italy. Fifteen isolated strains were studied for their different biodegradative capacities. Among these, 13 were able to grow on naphtha solvent. Ten were identified as Pseudomonas putida and three as Pseudomonas aureofaciens. Two other degraders were identified as Pseudomonas aeruginosa and Alcaligenes xylosoxidans subsp, denitrificans. Further molecular characterization of the isolates was carried out by randomly amplified polymorphic DNA analysis to ascertain that all the studied strains belonged to different haplotypes. The isolates were characterized for the presence of genes encoding for toluene dioxygenase, xylene monooxygenase and catechol 2,3-dioxygenase by polymerase chain reaction analysis and by Southern analysis. P, putida strain CM23, which showed homology with xylA,M, xylE and todC1C2BA genes, possessed multiple pathways which enabled the strain to grow on benzene, toluene and m-xylene.
Methylbenzene degradative pathway; RAPD; todC1C2BA-xylA, M-like genes
Settore AGR/16 - Microbiologia Agraria
Settore CHIM/06 - Chimica Organica
Settore BIO/19 - Microbiologia Generale
2000
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/179566
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