Interspecific introgression lines (ILs) have been used extensively in plants as their power to detect QTLs with small effects is higher compared to mapping populations having whole genome fragments segregating. In addition, interspecific ILs are a valuable tool for adding foreign DNA in "elite" varieties to improve their agronomic traits. Sterility as well as low levels of viability can affect the development of intespecific ILs despite that they were successfully obtained for tomato, watermelon and barley. Among Triticum species, two different Au and Am genomes derived from Triticum urartu and Triticum monococcum, respectively, were identified. Au and Am genomes share similar chromosomal organization; notwithstanding, significant molecular divergences have been assessed using sequence data and AFLP markers, supporting chimeric Au/Am chromosomes as a tool to dissect their genetic differences. The domesticated T. monococcum accessions L118 and the T. urartu accession ID388 differ in the grain content of Zn, Ca, carotenoids, tocols as well as in other important agronomic traits. We joined these hallmarks with IL properties to gain insights on the key loci controlling micronutrient content of kernel in diploid wheats. Offsprings obtained from crosses between T. urartu and T. monococcum were reported to be sterile, but rare fertile F1 plants were obtained by crossing the "elite" accession L118 with the wild accession ID388. From these fertile genotypes, ILs were obtained after several cycles of backcrossing using L118 as the recurrent parent. Afterwards, the ILs were fingerprinted using AFLP and previously mapped microsatellite markers, to characterize the introgressed fragment in each IL and to construct a panel of ILs carrying overlapping chromosome fragments of T. urartu. The phenotyping of the IL panel for some important traits associated with micronutrient content in kernel as well as statistical analyses searching contingent or transgressive QTLs are currently underway.
Mapping Quality Traits Associated with Grain Micronutrient Content in Diploid Wheats Using Interspecific Introgression Lines / A. Fricano, A. Brandolini, D. Boyer, A. Hidalgo, D. Erba, P. Sourdille, F. Salamini, P. Piffanelli. ((Intervento presentato al convegno 19th International Triticeae Mapping Initiative-3rd COST Action Tritigen tenutosi a Clermont-Ferrand, Francia nel 2009.
Mapping Quality Traits Associated with Grain Micronutrient Content in Diploid Wheats Using Interspecific Introgression Lines
A. Hidalgo;D. Erba;
2009
Abstract
Interspecific introgression lines (ILs) have been used extensively in plants as their power to detect QTLs with small effects is higher compared to mapping populations having whole genome fragments segregating. In addition, interspecific ILs are a valuable tool for adding foreign DNA in "elite" varieties to improve their agronomic traits. Sterility as well as low levels of viability can affect the development of intespecific ILs despite that they were successfully obtained for tomato, watermelon and barley. Among Triticum species, two different Au and Am genomes derived from Triticum urartu and Triticum monococcum, respectively, were identified. Au and Am genomes share similar chromosomal organization; notwithstanding, significant molecular divergences have been assessed using sequence data and AFLP markers, supporting chimeric Au/Am chromosomes as a tool to dissect their genetic differences. The domesticated T. monococcum accessions L118 and the T. urartu accession ID388 differ in the grain content of Zn, Ca, carotenoids, tocols as well as in other important agronomic traits. We joined these hallmarks with IL properties to gain insights on the key loci controlling micronutrient content of kernel in diploid wheats. Offsprings obtained from crosses between T. urartu and T. monococcum were reported to be sterile, but rare fertile F1 plants were obtained by crossing the "elite" accession L118 with the wild accession ID388. From these fertile genotypes, ILs were obtained after several cycles of backcrossing using L118 as the recurrent parent. Afterwards, the ILs were fingerprinted using AFLP and previously mapped microsatellite markers, to characterize the introgressed fragment in each IL and to construct a panel of ILs carrying overlapping chromosome fragments of T. urartu. The phenotyping of the IL panel for some important traits associated with micronutrient content in kernel as well as statistical analyses searching contingent or transgressive QTLs are currently underway.
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