This communication reports physiologic and structural characteristics of phage Z63-B2 active on Lactobacillus fermentum strain ATCC 9338; a comparison with phage Z63-B1, previously isolated from the same kind of environment (sourdoughs for bread-making) was discussed. Z63-B2 showed a multiplicative cycle with a burst size of 100 PFU per infectious centre and a latent period of 150 min. Its electrophoretical profile of proteins differed from that of phage Z63-B1 only for one minor protein. DNA fragments obtained with different Restriction enzymes resulted very similar and a high homology between the viruses was confirmed by hybridization tests. Two phages prove to be Variants each other. Resistant clones to phage Z63-B2 isolated after lysis of the host strain, showed costantly a different cell morphology and colony growth in comparison with the original host strain; however resistance was not imputable to a lysogenic state.

Characterization of Lactobacillus fermentum bacteriophage Z63-B2 / R. Foschino, E. Castiglioni, A. Galli. - In: ANNALI DI MICROBIOLOGIA ED ENZIMOLOGIA. - ISSN 0003-4649. - 48(1998), pp. 151-159.

Characterization of Lactobacillus fermentum bacteriophage Z63-B2

R. Foschino
Primo
;
1998

Abstract

This communication reports physiologic and structural characteristics of phage Z63-B2 active on Lactobacillus fermentum strain ATCC 9338; a comparison with phage Z63-B1, previously isolated from the same kind of environment (sourdoughs for bread-making) was discussed. Z63-B2 showed a multiplicative cycle with a burst size of 100 PFU per infectious centre and a latent period of 150 min. Its electrophoretical profile of proteins differed from that of phage Z63-B1 only for one minor protein. DNA fragments obtained with different Restriction enzymes resulted very similar and a high homology between the viruses was confirmed by hybridization tests. Two phages prove to be Variants each other. Resistant clones to phage Z63-B2 isolated after lysis of the host strain, showed costantly a different cell morphology and colony growth in comparison with the original host strain; however resistance was not imputable to a lysogenic state.
bacteriophage; cell morphology; colony morphology; Lactobacillus fermentum; resistant mutant; sour dough
Settore AGR/16 - Microbiologia Agraria
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/179058
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