Brain distribution pattern of "street" heroin metabolites (morphine and codeine) was investigated in two fatalities due to "acute narcotism". A suitable sample pretreatment prior to solid-phase-extraction was developed to achieve a good recovery of the analytes and to eliminate the interfering species. After derivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearity of the method were evaluated; LOD and LOQ were, respectively, 10ng/25ng for morphine and 5ng/10ng for codeine. This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found.

Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain / K. Guerrini, A. Argo, C. Borroni, D. Catalano, L. Dell'Acqua, F. Farè, P. Procaccianti, G. Roda, V. Gambaro. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 73:(2013 Jan 25), pp. 125-130. [10.1016/j.jpba.2012.04.001]

Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain

K. Guerrini
Primo
;
D. Catalano;L. Dell'Acqua;F. Farè;G. Roda
Penultimo
;
V. Gambaro
Ultimo
2013

Abstract

Brain distribution pattern of "street" heroin metabolites (morphine and codeine) was investigated in two fatalities due to "acute narcotism". A suitable sample pretreatment prior to solid-phase-extraction was developed to achieve a good recovery of the analytes and to eliminate the interfering species. After derivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearity of the method were evaluated; LOD and LOQ were, respectively, 10ng/25ng for morphine and 5ng/10ng for codeine. This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found.
codeine; heroin; morphine; post-mortem brain specimen
Settore CHIM/08 - Chimica Farmaceutica
25-gen-2013
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/179002
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