Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and >5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens
Analysis of allergenic components of a Parietaria judaica pollen extract by chromatographic methods for the evaluation of purification procedures / A. Bassoli, F. Chioccara, G. di Gregorio, B. Rindone, S. Tollari, P. Falagiani, G. Riva, E. Bolzacchini. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 444:C(1988), pp. 209-218. [10.1016/S0021-9673(01)94024-5]
Analysis of allergenic components of a Parietaria judaica pollen extract by chromatographic methods for the evaluation of purification procedures
A. BassoliPrimo
;S. Tollari;
1988
Abstract
Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and >5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
