Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and >5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens

Analysis of allergenic components of a Parietaria judaica pollen extract by chromatographic methods for the evaluation of purification procedures / A. Bassoli, F. Chioccara, G. di Gregorio, B. Rindone, S. Tollari, P. Falagiani, G. Riva, E. Bolzacchini. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 444:C(1988), pp. 209-218.

Analysis of allergenic components of a Parietaria judaica pollen extract by chromatographic methods for the evaluation of purification procedures

A. Bassoli
Primo
;
S. Tollari;
1988

Abstract

Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and >5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens
Allergens ; Chromatography Gel ; Chromatography High Pressure Liquid ; Isoelectric Focusing ; Plant Extracts ; Pollen ; Radioallergosorbent Test
Settore CHIM/06 - Chimica Organica
Settore CHIM/01 - Chimica Analitica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/177974
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