The interaction of daunorubicin with the self-complementary DNA fragment d(CGTACG) was studied by 31P NMR spectroscopy. The individual phosphates have been assigned for the nucleotide and the complex and signals from bound and free species in slow exchange at 19 degrees C were detected. In solution, the hexanucleotide binds two molecules of daunorubicin, which intercalate in the d(CG) sequence at both ends of the helix. Evidence for local deformations of the backbone at the sites of C5pG6, C1pG2 and G2pT3 phosphates is given. The binding constants for the stepwise equilibrium and the rate of dissociation of the intercalated duplex were also determined.

31P NMR study of daunorubicin-d(CGTACG) complex in solution Evidence of the intercalation sites / E. Ragg, R. Mondelli, C. Battistini, A. Garbesi, F. Colonna. - In: FEBS LETTERS. - ISSN 0014-5793. - 236:1(1988), pp. 231-234.

31P NMR study of daunorubicin-d(CGTACG) complex in solution Evidence of the intercalation sites

E. Ragg
Primo
;
R. Mondelli
Secondo
;
1988

Abstract

The interaction of daunorubicin with the self-complementary DNA fragment d(CGTACG) was studied by 31P NMR spectroscopy. The individual phosphates have been assigned for the nucleotide and the complex and signals from bound and free species in slow exchange at 19 degrees C were detected. In solution, the hexanucleotide binds two molecules of daunorubicin, which intercalate in the d(CG) sequence at both ends of the helix. Evidence for local deformations of the backbone at the sites of C5pG6, C1pG2 and G2pT3 phosphates is given. The binding constants for the stepwise equilibrium and the rate of dissociation of the intercalated duplex were also determined.
31P-NMR; Daunorubicin; DNA-drug interaction; Oligonucleotide
Settore CHIM/06 - Chimica Organica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/177656
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