A simple method for fractionating synthetic carrier ampholytes is reported, based on the principle of continuous-flow isoelectric focusing in gel-stabilized layers. An 8% ampholyte solution, encompassing the pH range 3-9.5, is separated into 12 fractions in a chamber filled with Sephadex G-100 by a continuous-flow technique. We are thus able to obtain ampholytes of narrow pH range, encompassing approximately 2 ph units, whose resolving power is comparable with that obtained with commercial Ampholine covering similar pH ranges.

Fractionation of carrier ampholytes for isoelectric focusing / E. Gianazza, M. Pagani, M. Luzzana, P.G. Righetti. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 109:2(1975 Jun 18), pp. 357-364.

Fractionation of carrier ampholytes for isoelectric focusing

E. Gianazza
Primo
;
1975

Abstract

A simple method for fractionating synthetic carrier ampholytes is reported, based on the principle of continuous-flow isoelectric focusing in gel-stabilized layers. An 8% ampholyte solution, encompassing the pH range 3-9.5, is separated into 12 fractions in a chamber filled with Sephadex G-100 by a continuous-flow technique. We are thus able to obtain ampholytes of narrow pH range, encompassing approximately 2 ph units, whose resolving power is comparable with that obtained with commercial Ampholine covering similar pH ranges.
Swine ; Animals ; Mucor ; Chickens ; Cattle ; Chymosin ; Electrophoresis, Polyacrylamide Gel ; Hydrogen-Ion Concentration ; Pepsin A ; Isoelectric Focusing
Settore BIO/10 - Biochimica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/176581
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 21
  • ???jsp.display-item.citation.isi??? ND
social impact