A simple method for fractionating synthetic carrier ampholytes is reported, based on the principle of continuous-flow isoelectric focusing in gel-stabilized layers. An 8% ampholyte solution, encompassing the pH range 3-9.5, is separated into 12 fractions in a chamber filled with Sephadex G-100 by a continuous-flow technique. We are thus able to obtain ampholytes of narrow pH range, encompassing approximately 2 ph units, whose resolving power is comparable with that obtained with commercial Ampholine covering similar pH ranges.
Fractionation of carrier ampholytes for isoelectric focusing / E. Gianazza, M. Pagani, M. Luzzana, P.G. Righetti. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 109:2(1975 Jun 18), pp. 357-364. [10.1016/S0021-9673(01)91808-4]
Fractionation of carrier ampholytes for isoelectric focusing
E. GianazzaPrimo
;
1975
Abstract
A simple method for fractionating synthetic carrier ampholytes is reported, based on the principle of continuous-flow isoelectric focusing in gel-stabilized layers. An 8% ampholyte solution, encompassing the pH range 3-9.5, is separated into 12 fractions in a chamber filled with Sephadex G-100 by a continuous-flow technique. We are thus able to obtain ampholytes of narrow pH range, encompassing approximately 2 ph units, whose resolving power is comparable with that obtained with commercial Ampholine covering similar pH ranges.
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