After myeloablative regimens, combined reinfusion of peripheral blood hematopoietic circulating progenitor cells (CPC) and bone marrow, yields a very rapid hematopoietic recovery. Therefore, based on the knowledge that CPC express the CD34 and CD33 differentiation antigen, we have developed a direct immunofluorescence flow cytometry assay to detect the peak of CPC in the peripheral blood of patients treated with high dose chemotherapy and growth factors. This assay, compared to CFU-GM assay, has the following advantages: 1) easy to do 2) standardized method 3) real time information on CPC number. This work illustrates the practical aspects of this assay and substantiate the widespread use of the CD34/33 flow cytometry assay to guide harvesting of circulating hematopoietic progenitors for autologous transplantation.

Quantization of CD34+ peripheral blood hematopoietic progenitors for autografting in cancer patients / M. Di Nicola, S. Siena, M. Bregni, F. Peccatori, M. Magni, F. Ravagnani, L. Zorzino, G. Bonadonna, A. Gianni. - In: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS. - ISSN 0391-3988. - 16 Suppl 5:5(1993 Dec), pp. 80-82.

Quantization of CD34+ peripheral blood hematopoietic progenitors for autografting in cancer patients

S. Siena;A. Gianni
1993

Abstract

After myeloablative regimens, combined reinfusion of peripheral blood hematopoietic circulating progenitor cells (CPC) and bone marrow, yields a very rapid hematopoietic recovery. Therefore, based on the knowledge that CPC express the CD34 and CD33 differentiation antigen, we have developed a direct immunofluorescence flow cytometry assay to detect the peak of CPC in the peripheral blood of patients treated with high dose chemotherapy and growth factors. This assay, compared to CFU-GM assay, has the following advantages: 1) easy to do 2) standardized method 3) real time information on CPC number. This work illustrates the practical aspects of this assay and substantiate the widespread use of the CD34/33 flow cytometry assay to guide harvesting of circulating hematopoietic progenitors for autologous transplantation.
Neoplasms; Leukapheresis; Hematopoietic Stem Cells; Antineoplastic Agents; Humans; Antigens, CD; Hematopoietic Stem Cell Transplantation; Flow Cytometry; Transplantation, Autologous; Colony-Forming Units Assay; Fluorescent Antibody Technique; Antigens, CD34
Settore MED/06 - Oncologia Medica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/176423
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