In two different cytolytic assays, dose-dependent inhibition of antibody-dependent cell-mediated cytotoxicity and natural killing by alpha 2-macroglobulin (alpha 2m) and its subunits was observed. Alpha 2m subunits were approximately four times more inhibitory in both assays than the native alpha 2m molecule on a molar basis. Analysis of trypsin protein esterase activity demonstrated that alpha 2 subunits were four times more active than the parent molecule.

Modulation of the immune response by plasma protease inhibitors. II. Alpha 2-macroglobulin subunits inhibit natural killer cell cytotoxicity and antibody-dependent cell-mediated cytotoxicity / P. Gravagna, E. Gianazza, P. Arnaud, M. Neels, E. W. Ades. - In: SCANDINAVIAN JOURNAL OF IMMUNOLOGY. - ISSN 0300-9475. - 15:1(1982 Jan), pp. 115-118. [10.1111/j.1365-3083.1982.tb00629.x]

Modulation of the immune response by plasma protease inhibitors. II. Alpha 2-macroglobulin subunits inhibit natural killer cell cytotoxicity and antibody-dependent cell-mediated cytotoxicity

E. Gianazza
Secondo
;
1982

Abstract

In two different cytolytic assays, dose-dependent inhibition of antibody-dependent cell-mediated cytotoxicity and natural killing by alpha 2-macroglobulin (alpha 2m) and its subunits was observed. Alpha 2m subunits were approximately four times more inhibitory in both assays than the native alpha 2m molecule on a molar basis. Analysis of trypsin protein esterase activity demonstrated that alpha 2 subunits were four times more active than the parent molecule.
Cytotoxicity, Immunologic ; Antibody-Dependent Cell Cytotoxicity ; Immunity, Cellular ; alpha-Macroglobulins ; Humans ; Dose-Response Relationship, Immunologic ; Trypsin Inhibitors ; Iodoacetamide ; Orosomucoid ; Dithiothreitol ; Protein Conformation ; Protease Inhibitors
Settore BIO/10 - Biochimica
gen-1982
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/176179
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