An expedient chemical synthesis of a 75mer peptide corresponding to the DNA binding domain (DBD, 227-301) of the human MafA leucine zipper transcription factor is reported. The application of microwave-assisted solid phase peptide synthesis (MW-SPPS) with a protocol modified respect to the standard one allowed obtaining the desired 75mer peptide in a short time with high quantity and optimal purity. MW-SPPS methodology was thus demonstrated as a valuable alternative to recombinant methods to obtain protein domains. Considering that recent findings suggest an involvement of MafA in the pathogenesis of diabetes mellitus, we also performed circular dichroism studies both on DBD folding and its interaction with MafA recognition element (MARE) on insulin enhancer. From our results, it was evicted that a disorder to order transition occurs after DBD interaction with insulin MARE which is mediated by specific structural elements on the N-terminus of the DBD.
Expedient chemical synthesis of 75mer DNA binding domain of MafA : an insight on its binding to insulin enhancer / S. Pellegrino, C. Annoni, A. Contini, F. Clerici, M.L. Gelmi. - In: AMINO ACIDS. - ISSN 0939-4451. - 43:5(2012 Nov), pp. 1995-2003.
|Titolo:||Expedient chemical synthesis of 75mer DNA binding domain of MafA : an insight on its binding to insulin enhancer|
PELLEGRINO, SARA (Primo)
ANNONI, CHIARA (Secondo)
CLERICI, FRANCESCA (Penultimo)
GELMI, MARIA LUISA (Ultimo)
|Parole Chiave:||MafA ; Leucine zipper ; Insulin enhancer ; Microwave-assisted solid phase peptide synthesis ; Circular dichroism|
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
|Data di pubblicazione:||nov-2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00726-012-1274-2|
|Appare nelle tipologie:||01 - Articolo su periodico|