A novel protocol for direct detection and localization of lectin binding sites on the surface of single sperm cells is presented. Fluorescence-conjugated lectins and single cell manipulation techniques were employed in this protocol. Advantages of the protocol include that sperm cell membranes are well protected and artifacts avoided by minimizing movement of the medium. Damage of the sperm membrane was reduced using a droplet-staining/washing method. Any morphological change may be readily recorded.

A reliable protocol for direct detection of lectin binding sites on the plasma membrane of a single living sperm cell in maize / M.-X. Sun, E. Kranz, A. Moscatelli, H.-Y. Yang, H. Lorz, M. Cresti. - In: SEXUAL PLANT REPRODUCTION. - ISSN 0934-0882. - 15:1(2002), pp. 53-55.

A reliable protocol for direct detection of lectin binding sites on the plasma membrane of a single living sperm cell in maize

A. Moscatelli;
2002

Abstract

A novel protocol for direct detection and localization of lectin binding sites on the surface of single sperm cells is presented. Fluorescence-conjugated lectins and single cell manipulation techniques were employed in this protocol. Advantages of the protocol include that sperm cell membranes are well protected and artifacts avoided by minimizing movement of the medium. Damage of the sperm membrane was reduced using a droplet-staining/washing method. Any morphological change may be readily recorded.
In vitro fertilization; Keywords Sperm cells; Lectin binding sites; Maize
Settore BIO/01 - Botanica Generale
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/15623
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 11
social impact