An experiment on Soft X-ray Contact Microscopy (SXCM) performed on Caenorhabditis elegans nematodes is discussed. This sample has been selected since it is a well studied case used as model in many biological contexts. The experiment has been performed using the iodine PALS laser source to generate pulsed soft X-rays from laser-plasma interaction, using molybdenum and gold as targets. Typical intensities on the targets exceeded 1014 W/cm 2. The SXCM imprints have been recorded on Polymethilmetacrylate (PMMA) photo resists which have been chemically developed and analyzed with an Atomic Force Microscope (AFM) operating in constant force mode. The use of error signal AFM images together with topography AFM images, did allow an easier recognition of biological patterns, and the identification of observed structures with internal organs. Several organs were identified in the SXCM images, including cuticle annuli, alae, pharynx, and three different types of cell nuclei. These are the first SXCM images of multi-cellular complex organisms.
X-ray microscopy and imaging of Caenorhabditis elegans nematode using a laser plasma pulsed x-ray source / G. Poletti, F. Orsini, J. Ullschmied, J. Skala, B. Kralikova, M. Pfeifer, C. Kadlec, T. Mocek, A. Prag, F. Cotelli, C. Lora Lamia, D. Batani, A. Bernardinello, T. Desai, A. Zullini. - 5196(2004), pp. 375-382. ((Intervento presentato al convegno Telescopes and instrumentation for solar astrophysics tenutosi a San Diego nel 2003.
X-ray microscopy and imaging of Caenorhabditis elegans nematode using a laser plasma pulsed x-ray source
G. PolettiPrimo
;F. OrsiniSecondo
;F. Cotelli;C. Lora Lamia;
2004
Abstract
An experiment on Soft X-ray Contact Microscopy (SXCM) performed on Caenorhabditis elegans nematodes is discussed. This sample has been selected since it is a well studied case used as model in many biological contexts. The experiment has been performed using the iodine PALS laser source to generate pulsed soft X-rays from laser-plasma interaction, using molybdenum and gold as targets. Typical intensities on the targets exceeded 1014 W/cm 2. The SXCM imprints have been recorded on Polymethilmetacrylate (PMMA) photo resists which have been chemically developed and analyzed with an Atomic Force Microscope (AFM) operating in constant force mode. The use of error signal AFM images together with topography AFM images, did allow an easier recognition of biological patterns, and the identification of observed structures with internal organs. Several organs were identified in the SXCM images, including cuticle annuli, alae, pharynx, and three different types of cell nuclei. These are the first SXCM images of multi-cellular complex organisms.
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