One of the most important issues in orthopaedic surgery is the loss of bone resulting from trauma, infections, tumors or congenital deficiencies. Nowadays, for the treatment of bone diseases a valid approach is the use of autologous cells able, alone or in combination with osteoconductive scaffold, to enhance the regeneration process of bone defect. Adipose-derived stem cells (ASCs), with their great availability and osteogenic potential may represent, in association with specific supports, a novel and efficient approach for bone regeneration. In this study, autologous rabbit ASCs were used for the treatment of a full-thickness bone defects in the proximal epiphysis of tibia of twelve New Zealand rabbits as follows: sham (just defect), insertion of hydroxyapatite disk (HA), of ASCs alone (ASCs), and of hydroxyapatite-ASCs seeded-disk (HA-ASCs). Each ASCs population was tested in vitro: all of them show a high proliferation rate with a doubling time of 56.5±16.9 hours, a marked clonogenic ability (3.0±1.6%) and osteogenic differentiation potential evaluated by extracellular calcified matrix deposition (161.6±77.9% increase of osteo- vs undifferentiated ASCs). Eight-weeks after surgery, the macroscopic analyses show satisfactory filling of the lesion without any significant differences in term of stiffness between groups treated with or without cells (p>0.05). In both the scaffold-treated groups, a good osteointegration was radiographically observed. Even if HA was not completely reabsorbed, ASCs-loaded HA displayed a more efficient scaffold resorption than the unloaded ones. Furthermore, histological analyses show the osteogenic abilities of the scaffold-treated defects with respect to the scaffold cell-free samples, and in particular, the new formed bone was more mature and similar to native bone in presence of ASCs. These results indicate that autologous ASCs-hydroxyapatite construct is a potential treatment for the regeneration of bone defects.
Critical bone defect repair by autologous rabbit adipose-derived stem cells (ASCS) loaded onto hydroxyapatite scaffold / E. Arrigoni, L. De Girolamo, D. Stanco, C. Domeneghini, A.T. Brini. ((Intervento presentato al 3. convegno International Congress on Stem Cells and Tissue Formation tenutosi a Dresden nel 2010.
|Titolo:||Critical bone defect repair by autologous rabbit adipose-derived stem cells (ASCS) loaded onto hydroxyapatite scaffold|
ARRIGONI, ELENA (Primo)
DE GIROLAMO, LAURA (Secondo)
DOMENEGHINI, CINZIA MARIA (Penultimo)
BRINI, ANNA TERESA (Ultimo)
|Data di pubblicazione:||lug-2010|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
Settore VET/01 - Anatomia degli Animali Domestici
|Enti collegati al convegno:||Deutsche Gesellschaft für Hämatologie und Onkologie (DGHO)|
|Citazione:||Critical bone defect repair by autologous rabbit adipose-derived stem cells (ASCS) loaded onto hydroxyapatite scaffold / E. Arrigoni, L. De Girolamo, D. Stanco, C. Domeneghini, A.T. Brini. ((Intervento presentato al 3. convegno International Congress on Stem Cells and Tissue Formation tenutosi a Dresden nel 2010.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|