The dissociation constants of epiboxidine 2 and a series of bases active at neuronal nicotinic acetylcholine receptors were determined by means of potentiometric and electrophoretic methods, which gave values in good agreement. Although showing different features, the two techniques are complementary for dissociation constant determinations. The choice of the most suitable method is guided by the available amount of sample, its purity, and the time needed for the analysis. The experimental values were compared with the predictions obtained with ACD/pK(a) DB software.

Determination of acid dissociation constants of compounds active at neuronal nicotinic acetylcholine receptors by means of electrophoretic and potentiometric techniques / G. Roda, C. Dallanoce, G. Grazioso, V. Liberti, M. De Amici. - In: ANALYTICAL SCIENCES. - ISSN 0910-6340. - 26:1(2010), pp. 51-54. [10.2116/analsci.26.51]

Determination of acid dissociation constants of compounds active at neuronal nicotinic acetylcholine receptors by means of electrophoretic and potentiometric techniques

G. Roda;C. Dallanoce;G. Grazioso;V. Liberti;M. De Amici
2010

Abstract

The dissociation constants of epiboxidine 2 and a series of bases active at neuronal nicotinic acetylcholine receptors were determined by means of potentiometric and electrophoretic methods, which gave values in good agreement. Although showing different features, the two techniques are complementary for dissociation constant determinations. The choice of the most suitable method is guided by the available amount of sample, its purity, and the time needed for the analysis. The experimental values were compared with the predictions obtained with ACD/pK(a) DB software.
capillary-zone-electrophoresis; PK(A) values; drugs; water
Settore CHIM/08 - Chimica Farmaceutica
Nuovi ligandi selettivi dei recettori nicotinici neuronali omomerici ed eteromerici: progettazione, sintesi, indagine bio-farmacologica e relazioni struttura/attività.
ANALYTICAL SCIENCES
Article (author)
File in questo prodotto:
File Dimensione Formato  
26_51.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 308.95 kB
Formato Adobe PDF
308.95 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/145818
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 18
  • ???jsp.display-item.citation.isi??? 20
social impact