Performances and advantages of different high-resolution microscopy techniques are compared. These include Soft X-ray Contact Microscopy (SXCM), Atomic Force Microscopy (AFM), Focused Ion Beam (FIB) and Transmission Electron Microscopy (TEM). It is shown that they allow complementary approaches to imaging of biological objects. These techniques have been used to image the same type of cells (Saccharomyces cerevisiae) thus providing a common benchmark. In particular it is shown that the novel FIB technique allows easy target cell selection, fast operation, high resolution, 3D imaging and sample manipulation during imaging.

High resolution microscopy techniques for the analysis of biological samples : a comparison / M. Milani, M. Ballerini, D. Batani, F. Squadrini, F. Cotelli, C. Lora Lamia Donin, G. Poletti, A. Pozzi, K. Eidmann, A. Stead, G. Lucchini. - In: THE EUROPEAN PHYSICAL JOURNAL. APPLIED PHYSICS. - ISSN 1286-0042. - 26:2(2004), pp. 123-131.

High resolution microscopy techniques for the analysis of biological samples : a comparison

F. Cotelli;C. Lora Lamia Donin;
2004

Abstract

Performances and advantages of different high-resolution microscopy techniques are compared. These include Soft X-ray Contact Microscopy (SXCM), Atomic Force Microscopy (AFM), Focused Ion Beam (FIB) and Transmission Electron Microscopy (TEM). It is shown that they allow complementary approaches to imaging of biological objects. These techniques have been used to image the same type of cells (Saccharomyces cerevisiae) thus providing a common benchmark. In particular it is shown that the novel FIB technique allows easy target cell selection, fast operation, high resolution, 3D imaging and sample manipulation during imaging.
Settore BIO/05 - Zoologia
Settore BIO/06 - Anatomia Comparata e Citologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/14568
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